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SINGLE CT VALUE FOR COVID TEST

SINGLE CT VALUE FOR COVID TEST

Context

Recently Indian Council of Medical Research decided a single Ct value cut-off for Covid-19 test.

Details

  • All patients with a Ct value less than 35 may be considered as positive while those with a Ct value above 35 may be considered as negative.
  • Ct value refers to the number of cycles after which the virus can be detected.
  • In RT-PCR (Reverse Transcription Polymerase Chain Reaction) test, RNA (Ribonucleic acid) is extracted from the swab collected from the patient.
  • It is then converted into DNA (Deoxyribonucleic acid), which is then amplified.
  • As per the ICMR advisory, the Ct value of an RT-PCR reaction is the number of cycles at which fluorescence of the PCR product is noticeable over and above the background signal.

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